Journal: International Journal of Molecular Medicine

Article Title: The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

doi: 10.3892/ijmm.2018.3480

Figure Lengend Snippet: The bone morphogenetic protein (BMP) signaling pathway is activated in intestinal epithelial cells and intraepithelial lymphocytes (IELs) following ischemia/reperfusion (I/R). (A) The level of BMP4 protein (red) expression significantly increased in the mid-to-distal villus region after 6 h of I/R, according to immunofluorescence staining. (B) The expression levels of the type I BMP receptor and phosphorylated nuclear factor (p-NF)-κB were both significantly increased after 6 h of I/R in IELs. * P<0.05, 3-4 mice/group; the results are representative of three independent experiments.

Article Snippet: Recombinant BMP4 protein (cat. no. 315-27; 30 ng/ml) and recombinant murine IL-7 protein (cat. no. 217-17; 2 ng/ml) were both purchased from ProteinTech Group, Inc. Pyrrolidine dithiocarbamate (PDTC; cat. no. S1808; 30 ng/ml), an inhibitor of NF-κB, was purchased from Beyotime Institute of Biotechnology (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining

Journal: International Journal of Molecular Medicine

Article Title: The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

doi: 10.3892/ijmm.2018.3480

Figure Lengend Snippet: Activation of nuclear factor (NF)-κB signaling by bone morphogenetic protein (BMP)4 stimulation in intraepithelial lymphocytes (IELs). Flow cytometry determined the expression of the BMP type I receptor and phosphorylated NF-κB in IELs in culture. Following treatment with BMP4 for 6 h, the expression of the BMP type I receptor and phosphorylated NF-κB was significantly increased compared with that in the control group. NOGGIN partially decreased the expression of the BMP type I receptor, as well as NF-κB transcriptional activity. The results are representative of three independent experiments. P<0.05.

Article Snippet: Recombinant BMP4 protein (cat. no. 315-27; 30 ng/ml) and recombinant murine IL-7 protein (cat. no. 217-17; 2 ng/ml) were both purchased from ProteinTech Group, Inc. Pyrrolidine dithiocarbamate (PDTC; cat. no. S1808; 30 ng/ml), an inhibitor of NF-κB, was purchased from Beyotime Institute of Biotechnology (Wuhan, China).

Techniques: Activation Assay, Flow Cytometry, Expressing, Control, Activity Assay

Journal: International Journal of Molecular Medicine

Article Title: The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

doi: 10.3892/ijmm.2018.3480

Figure Lengend Snippet: Bone morphogenetic protein (BMP)4 induces intraepithelial lymphocytes (IELs) to undergo apoptosis. Intestinal IELs were examined by flow cytometry for markers of apoptosis (FITC-Annexin V and PI). FITC-Annexin V + /PI + indicates late apoptosis, FITC-Annexin V + /PI − indicates early apoptosis, and FITC-Annexin V − /PI − indicates live cells. The extent of apoptosis of IELs after treatment with BMP4 for 6 h was then determined. The expression of FITC-Annexin V + /PI + IELs in the BMP4 group was significantly higher compared with that in the control group, but these effects were decreased by treatment with NOGGIN or pyrrolidine dithiocarbamate (PDTC). P<0.05, 3-4 mice/group; each experiment was repeated three times.

Article Snippet: Recombinant BMP4 protein (cat. no. 315-27; 30 ng/ml) and recombinant murine IL-7 protein (cat. no. 217-17; 2 ng/ml) were both purchased from ProteinTech Group, Inc. Pyrrolidine dithiocarbamate (PDTC; cat. no. S1808; 30 ng/ml), an inhibitor of NF-κB, was purchased from Beyotime Institute of Biotechnology (Wuhan, China).

Techniques: Flow Cytometry, Expressing, Control

Journal: International Journal of Molecular Medicine

Article Title: The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

doi: 10.3892/ijmm.2018.3480

Figure Lengend Snippet: Inhibition of bone morphogenetic protein (BMP)4 induces the apoptosis of intraepithelial lymphocytes (IELs) that has been stimulated by interleukin (IL)-7. Flow cytometry and apoptosis markers (FITC-Annexin V and PI) were used to examine IEL apoptosis after treatment with BMP4, IL-7 or BMP4 + IL-7 for 6 h. The expression of FITC-Annexin V + /PI + IELs in the IL-7 treatment group was significantly lower compared with that in the control group, whereas the expression of FITC-Annexin V + /PI + IELs in the BMP4 treatment group was significantly higher compared with that in the control group. However, the expression of FITC-Annexin V + /PI + IELs in the BMP4 + IL-7 treatment group exhibited no changes compared with the control group. Each experiment was repeated three times; 4-5 mice/group. P<0.05.

Article Snippet: Recombinant BMP4 protein (cat. no. 315-27; 30 ng/ml) and recombinant murine IL-7 protein (cat. no. 217-17; 2 ng/ml) were both purchased from ProteinTech Group, Inc. Pyrrolidine dithiocarbamate (PDTC; cat. no. S1808; 30 ng/ml), an inhibitor of NF-κB, was purchased from Beyotime Institute of Biotechnology (Wuhan, China).

Techniques: Inhibition, Flow Cytometry, Expressing, Control

Journal: International Journal of Molecular Medicine

Article Title: The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

doi: 10.3892/ijmm.2018.3480

Figure Lengend Snippet: Interleukin (IL)-7 downregulates the bone morphogenetic protein (BMP) signaling pathway in intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs). (A) Western blot analysis was used to determine the expression of BMP4 in IEC-6 cells following treatment with IL-7 for 6 h. The expression of BMP4 was significantly decreased compared with that in the control group. (B) Flow cytometry, was used to detect the phosphorylation of nuclear factor (NF)-κB following treatment with IL-7 for 6 h. The expression of phosphorylated NF-κB was significantly decreased compared with that in the control group. Each experiment was repeated three times; 4-5 mice/group. * P<0.05 vs. control. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Recombinant BMP4 protein (cat. no. 315-27; 30 ng/ml) and recombinant murine IL-7 protein (cat. no. 217-17; 2 ng/ml) were both purchased from ProteinTech Group, Inc. Pyrrolidine dithiocarbamate (PDTC; cat. no. S1808; 30 ng/ml), an inhibitor of NF-κB, was purchased from Beyotime Institute of Biotechnology (Wuhan, China).

Techniques: Western Blot, Expressing, Control, Flow Cytometry, Phospho-proteomics

Journal: International Journal of Molecular Medicine

Article Title: The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

doi: 10.3892/ijmm.2018.3480

Figure Lengend Snippet: Bone morphogenetic protein (BMP)4 regulates the interleukin (IL)-7α/CD127 signaling pathway in intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs). (A) Western blot analysis determined the expression of IL-7 in IEC-6 cells following treatment with BMP4 for 6 h. The IL-7 level was significantly decreased compared with that in the control group. (B) Flow cytometry, was used to detect the expression of CD127 and phosphorylated signal transducer and activator of transcription (STAT)5 proteins following treatment with BMP4 for 6 h. The levels of CD127 and phosphorylated STAT5 proteins were significantly decreased compared with those in the control group. Each experiment was repeated three times; 4-5 mice/group. * P<0.05 for control vs. treatment with BMP4 alone; ** P<0.05 for control vs. the BMP4 + NOGGIN group. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Recombinant BMP4 protein (cat. no. 315-27; 30 ng/ml) and recombinant murine IL-7 protein (cat. no. 217-17; 2 ng/ml) were both purchased from ProteinTech Group, Inc. Pyrrolidine dithiocarbamate (PDTC; cat. no. S1808; 30 ng/ml), an inhibitor of NF-κB, was purchased from Beyotime Institute of Biotechnology (Wuhan, China).

Techniques: Western Blot, Expressing, Control, Flow Cytometry

Journal: The FASEB Journal

Article Title: Low‐Intensity Pulsed Ultrasound Promotes Osteogenesis in Porous Titanium Alloys Through miR ‐1187/ BMP4 Pathway

doi: 10.1096/fj.202403395RR

Figure Lengend Snippet: miR‐1187 directly targets BMP4. (A) The predicted binding site between miR‐1187 and BMP4 by bioinformatics analysis. (B) The luciferase activity of the BMP4‐WT and BMP4‐MUT in MC3T3‐E1 cells treated with miR‐1187 mimics or NC. (C) mRNA expression of Bmp4 was significantly downregulated in the miR‐1187 mimics‐transfected group. (D) Western blot analysis for the expression of BMP4 protein in miR‐1187 mimics and inhibitor‐transfected MC3T3‐E1 cells on Pti. (E) ELISA assay analysis for the expression of supernatant BMP4 secreted protein in miR‐1187 mimics and inhibitor‐transfected MC3T3‐E1cells on Pti. All values represent means ± SD.( n = 3). * p < 0.05, ** p < 0.01.

Article Snippet: BMP4 protein levels in the supernatant were quantified using a mouse BMP‐4 ELISA kit (EK0316, Boster, China) following the manufacturer's protocol.

Techniques: Binding Assay, Luciferase, Activity Assay, Expressing, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: The FASEB Journal

Article Title: Low‐Intensity Pulsed Ultrasound Promotes Osteogenesis in Porous Titanium Alloys Through miR ‐1187/ BMP4 Pathway

doi: 10.1096/fj.202403395RR

Figure Lengend Snippet: BMP4 positively regulates osteogenic differentiation in MC3T3‐E1 cells. (A) Level of BMP4 decreased after transfected with different siRNA‐BMP4, si‐BMP4‐785 was identified to have the highest transfection efficiency. (B) BMP4 transcript levels was determined by RT‐PCR after transfected with overexpression plasmids of BMP4. (C) qRT‐PCR analysis. Level of Col‐1 , Alp , Runx2 , and Ocn mRNA expression was significantly downregulated in BMP4 silencing. (D) qRT‐PCR analysis. Level of Col‐1 , Alp , Runx2 , and Ocn mRNA expression was significantly upregulated in BMP4 overexpression. (E) Western blotting. Expression of BMP4 protein and osteogenesis‐related proteins COL‐1, ALP, RUNX2 as well as statistical analysis after transfecting si‐BMP4. (F) Western blotting. Expression of BMP4 protein and osteogenesis‐related proteins COL‐1, ALP, RUNX2 as well as statistical analysis after transfecting pCDNA3.1‐BMP4. (G) ALP activity detection on day 7 in si‐BMP4 and pCDNA3.1‐BMP4 transfected MC3T3‐E1cells. (H) ALP staining on day 10 in si‐BMP4 and pCDNA3.1‐BMP4 transfected MC3T3‐E1cells (scale bar = 500 μm). (I) Alizarin red S staining and quantitative analysis of Alizarin Red S accumulation on day 21 in si‐BMP4 and pCDNA3.1‐BMP4 transfected MC3T3‐E1cells (scale bar = 200 μm/100 μm), the black arrow indicate the magnified area, the magnified pictures were marked within the square frame in the image. All values represent means ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: BMP4 protein levels in the supernatant were quantified using a mouse BMP‐4 ELISA kit (EK0316, Boster, China) following the manufacturer's protocol.

Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Over Expression, Quantitative RT-PCR, Expressing, Western Blot, Activity Assay, Staining

Journal: The FASEB Journal

Article Title: Low‐Intensity Pulsed Ultrasound Promotes Osteogenesis in Porous Titanium Alloys Through miR ‐1187/ BMP4 Pathway

doi: 10.1096/fj.202403395RR

Figure Lengend Snippet: LIPUS promotes osteogenic differentiation of MC3T3‐E1 through inhibiting miR‐1187 and upregulating BMP4. (A) qRT‐PCR analysis. Effect of daily LIPUS stimulation on mRNA expression of Bmp4. (B) ELISA. Supernatant BMP4 secreted by cultured MC3T3‐E1 cells was determined by ELISA assay. (C) Western blotting. BMP4 level was analyzed by western blot after 7 days LIPUS treatment. (D) Expression of osteogenesis‐related mRNA was analyzed by qRT‐PCR after si‐BMP4 or si‐bmp4 + LIPUS treatment. (E) Expression of osteogenesis‐related protein was analyzed by western blot after si‐BMP4 or si‐BMP4 + LIPUS treatment. (F) ALP activity detection on day 7 in si‐BMP4 and si‐BMP4 + LIPUS‐treated MC3T3‐E1 cells. (G) Bmp4 and osteogenesis‐related genes Col‐1, Alp, Runx2, and Ocn expression in MC3T3‐E1 cells on Pti as indicated treatment by qRT‐PCR. (H) ALP activity in MC3T3‐E1 cells on Pti on day 7 as indicated treatment. (I) Expression of BMP4 and osteogenesis‐related proteins including COL‐1, ALP, RUNX2 in MC3T3‐E1 cells on Pti as indicated treatment by Western blot analysis. All values represent means ± SD ( n = 3). Expression of osteogenesis‐related protein was analyzed by western blot (E1) and quantitative analysis (E2) after si‐BMP4, LIPUS or si‐BMP4+LIPUS treated. Expression of BMP4 and osteogenesis‐ related proteins including COL‐ 1, ALP, RUNX2 in MC3T3‐ E1 cells on Pti as indicated treatment by Western blot analysis(I)andquantitative analysis (J). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: BMP4 protein levels in the supernatant were quantified using a mouse BMP‐4 ELISA kit (EK0316, Boster, China) following the manufacturer's protocol.

Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Western Blot, Activity Assay

Journal: The FASEB Journal

Article Title: Low‐Intensity Pulsed Ultrasound Promotes Osteogenesis in Porous Titanium Alloys Through miR ‐1187/ BMP4 Pathway

doi: 10.1096/fj.202403395RR

Figure Lengend Snippet: LIPUS regulates bone formation in vivo. (A) Study plan of the vitro study. (B) qRT‐PCR analysis of mRNA expression of Bmp4 as well as other osteogenesis‐related gene in the de novo bone of different groups. B1: MRNA expression of Bmp4; B2, B3: Osteogenesis‐related gene including Col‐1, Alp, Runx2, and Ocn at 4 weeks and 8 weeks respectively. (C) Micro‐CT analysis. C1: 3D reconstruction of the bone defect area at week 4 and 8. More bone ingrowth is observed as time increases in each group. The white part is the scaffold and the red part is the bone tissue. C2: The POF values for the Pti at 4 and 8 weeks. The POF values differ significantly between the LIPUS and control group, between the si‐BMP4 and control group, as well as between the si‐BMP4 and si‐BMP4 + LIPUS group. (D) D1: The representative merged images of fluorescent double labeling of calcein(green color) and xylenol orange(orange color). The red square indicate the magnified area, the magnified pictures were marked within the white square frame in the image. (scale bar = 200 μm/100 μm). D2: The MAR of the four groups at 4 and 8 weeks. All values represent means ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: BMP4 protein levels in the supernatant were quantified using a mouse BMP‐4 ELISA kit (EK0316, Boster, China) following the manufacturer's protocol.

Techniques: In Vivo, Quantitative RT-PCR, Expressing, Micro-CT, Control, Labeling

Journal: The FASEB Journal

Article Title: Low‐Intensity Pulsed Ultrasound Promotes Osteogenesis in Porous Titanium Alloys Through miR ‐1187/ BMP4 Pathway

doi: 10.1096/fj.202403395RR

Figure Lengend Snippet: The schematic of LIPUS promotes osteogenesis of porous titanium alloys through inhibiting miR‐1187 and upregulating BMP4.

Article Snippet: BMP4 protein levels in the supernatant were quantified using a mouse BMP‐4 ELISA kit (EK0316, Boster, China) following the manufacturer's protocol.

Techniques:

Journal: bioRxiv

Article Title: Respiratory Airway Secretory Cells act as Immune Sentinels in Human Distal Airways

doi: 10.1101/2025.03.24.644887

Figure Lengend Snippet: a , Schematic of proximal-distal patterning in developing human lung with representative lineage markers. b , Differentiation protocol workflow from human pluripotent stem cells (hPSCs) to distal lung organoids (d-LOs) via anterior foregut endoderm (AFE) spheroids dissociation. c , Immunofluorescence staining of NKX2-1, SOX2 and SOX9 in proximal (p-LO) and distal (d-LO) lung organoids at day 8. White arrows denote SOX2 bright NKX2-1 dim cells; arrowheads indicate SOX9 bright NKX2-1 bright cells. Scale bars, 20 μm. Representative images from three biologically independent experiments. d , Quantitative PCR analysis of NKX2-1 , SOX9 and SOX2 expression in AFE spheroids, d-LOs and p-LOs. Data presented as mean ± s.e.m. (n=3 biological replicates). P -values were calculated using two-tailed Student’s t -test with Welch’s correction. e , UMAP with annotations of different cell types from epithelial cell subclusters. f , Individual conditions of epithelial subclusters shown in ( e ). g , Proportional distribution of cell types between d-LO and p-LO. h , Violin plots showing expression levels of proximal and distal markers in p-LPC and d-LPC cell populations from d-LO and p-LO.

Article Snippet: Detached anterior foregut spheroids (days 5–7) were split into two groups: p-LO: Spheroids were embedded in growth factor-reduced Matrigel (Corning, 354230) and cultured in LPC medium (20 ng/ml BMP4 [MCE, HY-P7007], 10 ng/ml FGF7/KGF [MCE, HY-P70597], 10 ng/ml FGF10 [MCE, HY-P70695], 3 μM CHIR99021, 20 μM DAPT [MCE, HY-13027], and 50 nM retinoic acid [Sigma, R2625]). d-LO: Spheroids were washed with PBS, dissociated into single cells with Accutase, pelleted (300 × g, 5 min), and resuspended in growth factor-reduced Matrigel.

Techniques: Immunofluorescence, Staining, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

Journal: bioRxiv

Article Title: Respiratory Airway Secretory Cells act as Immune Sentinels in Human Distal Airways

doi: 10.1101/2025.03.24.644887

Figure Lengend Snippet: a , Differentiation protocol workflow for generating proximal lung organoids (p-LOs) from hPSCs. b , Bright-field images comparing organoid morphology under untreated versus dissociation conditions during lung progenitor cell (LPC) induction. Scale bars, 500 μm (far left and middle two panels), 200 μm (right panels). Representative of three biologically independent experiments. c , Phase-contrast images of day 8 p-LOs and d-LOs counterstained with Hoechst. Scale bars, 500 μm. d , Organoid yield quantification (number per Matrigel droplet). e , Organoid diameter measurements. f , Representative flow cytometry analysis of NKX2-1 expression in p-LOs versus d-LOs. n = 3 independent experiments. g , h , Percentage ( g ) and mean fluorescence intensity (MFI) ( h ) of NKX2-1 + cells. i , qPCR analysis of lung-specific and endodermal lineage markers in p-LOs and d-LOs derived from hESC lines (H1, H9) and induced pluripotent stem cells (hiPSCs). d , e , g , h , i , Data are presented as mean ± s.e.m. (n=3 biological replicates). P -values calculated using two-tailed Student’s t -test.

Article Snippet: Detached anterior foregut spheroids (days 5–7) were split into two groups: p-LO: Spheroids were embedded in growth factor-reduced Matrigel (Corning, 354230) and cultured in LPC medium (20 ng/ml BMP4 [MCE, HY-P7007], 10 ng/ml FGF7/KGF [MCE, HY-P70597], 10 ng/ml FGF10 [MCE, HY-P70695], 3 μM CHIR99021, 20 μM DAPT [MCE, HY-13027], and 50 nM retinoic acid [Sigma, R2625]). d-LO: Spheroids were washed with PBS, dissociated into single cells with Accutase, pelleted (300 × g, 5 min), and resuspended in growth factor-reduced Matrigel.

Techniques: Flow Cytometry, Expressing, Fluorescence, Derivative Assay, Two Tailed Test

Journal: Toxicological Sciences

Article Title: Bone morphogenetic protein 4 is involved in cadmium-associated bone damage

doi: 10.1093/toxsci/kfac121

Figure Lengend Snippet: Effects of CdCl 2 on BMP/SMAD pathway. The hBMSCs were exposed to 0, 2.5, or 5.0 μM CdCl 2 and subjected to osteogenic differentiation for 14 days. A and C, Western blots were performed, and (B and D) relative protein levels of BMP-2, BMP-4, BMP-6, BMP-7, SMAD1, p-SMAD1, SMAD4, SMAD5, and p-SMAD1/5/9 were determined. E and F, Expression levels of BMP-2 and BMP-4 were detected by ELISA. These are the results from 3 independent biological replicates experiments (mean ± SD, n = 3). * p < .05, ** p < .01, different from hMSCs in the absence of CdCl 2 . Abbreviations: BMP, bone morphogenetic protein; hBMSC, human bone marrow mesenchymal stem cell.

Article Snippet: For BMP-4 groups, the medium included 50 ng/ml of recombinant BMP-4 (MedChemexpress).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Toxicological Sciences

Article Title: Bone morphogenetic protein 4 is involved in cadmium-associated bone damage

doi: 10.1093/toxsci/kfac121

Figure Lengend Snippet: Association among Cd, BMP-4, and osteoporosis. The mediating role of plasma BMP-4 ligand in the relationship between U-Cd and occurrence of osteoporosis. Causal mediation analysis estimated the indirect effect of U-Cd on osteoporosis through plasma BMP-4 ligand. Abbreviations: BMP, bone morphogenetic protein; CI, confidence interval; DE, direct effect; IE, indirect effect; PM, proportion mediated; U-Cd, urinary cadmium.

Article Snippet: For BMP-4 groups, the medium included 50 ng/ml of recombinant BMP-4 (MedChemexpress).

Techniques: Clinical Proteomics

Journal: Toxicological Sciences

Article Title: Bone morphogenetic protein 4 is involved in cadmium-associated bone damage

doi: 10.1093/toxsci/kfac121

Figure Lengend Snippet: The osteogenic effect of BMP-4 on hBMSCs. The hBMSCs were treated with BMP-4 (50 ng/ml) and/or CdCl 2 (5.0 μM) for 14 days. A, The BMP-4 levels detected by ELISA. B, Mineralization was measured using Alizarin Red S staining. C, Western blots were performed. D, Relative protein levels of Runx2, OSX, SMAD4, and p-SMAD1/5/9 complex were determined. These are the results from 3 independent biological replicates experiments (mean ± SD, n = 3). ∗ p < .05, ** p < .01. Abbreviations: BMP, bone morphogenetic protein; hBMSC, human bone marrow mesenchymal stem cell.

Article Snippet: For BMP-4 groups, the medium included 50 ng/ml of recombinant BMP-4 (MedChemexpress).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Western Blot

Journal: Toxicological Sciences

Article Title: Bone morphogenetic protein 4 is involved in cadmium-associated bone damage

doi: 10.1093/toxsci/kfac121

Figure Lengend Snippet: The effects of BMP-4 knockdown on osteogenic differentiation and SMADs. After si-BMP-4 transfect the hBMSCs were osteogenic induction for 14 days. A, Mineralization was measured using Alizarin Red S staining. B, Western blots were performed. C, Relative protein levels of BMP-4, Runx2, OSX, SMAD4, and p-SMAD1/5/9 complex were determined. These are the results from 3 independent biological replicates experiments (mean ± SD, n = 3). ** p < .01.

Article Snippet: For BMP-4 groups, the medium included 50 ng/ml of recombinant BMP-4 (MedChemexpress).

Techniques: Knockdown, Staining, Western Blot

Journal: Redox Biology

Article Title: Endothelial TET2 regulates the white adipose browning and metabolism via fatty acid oxidation in obesity

doi: 10.1016/j.redox.2023.103013

Figure Lengend Snippet: Endothelial TET2 modulates lipolysis through secretion of BMP4. A , Diagram of in vivo WAT explants assay. B and C , FFA level of SAT (B) and VAT (C) obtained from WT or TET2 EC−KO mice at the time of 0 min or 120 min (n = 6 mice/group). D , Diagram of co-culture of bEnd.3 and adipocytes. E , qPCR analysis of bEnd.3 after overexpressing TET2 by adenovirus. n = 3/group. F, Representative images of oil red O staining in adipocytes after co-culturing with TET2 overexpressed bEnd.3. Scale bars = 50 μm. G , qPCR analysis of lipolysis-related genes in adipocytes after co-culturing with TET2 overexpressed bEnd.3. n = 3/group. H , Cell culture supernatants of HUVECs transfected with ctrl siRNA or TET2 siRNA were detected by human adipokine array kit. I , Relative mean pixel density of H was calculated. n = 3/group. J , Relative mRNA levels of BMP4 in HUVECs transfected with Ctrl siRNA or TET2 siRNA. n = 3/group. K , Serum BMP4 level of WT and TET2 EC−KO mice with HFD for 16 weeks. n = 6 mice/group. L , Representative images of adipocyte morphology (H&E), microvessels (IB4, red), UCP1 staining in SAT from WT or TET2 EC−KO mice with or without local injection of BMP4 in CL316243-treated model. M , Quantification of L. n = 6 mice/group. All data are mean ± SEM. E, G, I, J, K, unpaired Student's t -test. B, C, M, Two-way ANOVA with Bonferroni post hoc test. * P < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Insulin, BMP4 and global DNA methylation levels were measured by murine INS ELISA kits (Cat#YCQZ-10385, YC Bio), murine BMP4 ELISA kits (Cat#EK0316, Boster), and Global DNA Methylation–LINE-1 Kit (Cat#55017, Active Motif) respectively.

Techniques: In Vivo, Co-Culture Assay, Staining, Cell Culture, Transfection, Injection

Journal: Redox Biology

Article Title: Endothelial TET2 regulates the white adipose browning and metabolism via fatty acid oxidation in obesity

doi: 10.1016/j.redox.2023.103013

Figure Lengend Snippet: NRF2 recruits TET2 to regulate FAO. A , Binding sites of TET2 and NRF2 were predicted using Gramm-X. Blue, TET2; Cyan, NRF2; Red, binding sites. B and C , Lysate of HUVECs was immunoprecipitated with anti-TET2 antibody (B) or anti-NRF2 antibody (C) and then immunoblotted with the indicated antibodies. D , Schematic illustration of different plasmids of TET2 and NRF2. E and F , TET2-His and NRF2-Flag were ectopically expressed in HEK293T and the lysate of HEK293T was immunoprecipitated with anti-His (E) antibody or anti-Flag antibody (F). G , NRF2 was ectopically expressed with different domains of TET2 HEK293T and the lysate of HEK293T was immunoprecipitated with anti-Flag antibody. H , The 5-mC levels of HUVECs transfected with or without TET2 siRNA. n = 3/group. I , The methylation status of BMP4 and CPT1A promoter in HUVECs treated with AdNC or AdTET2. J , Predict binding sites of NRF2 in the promoters of CPT1A and BMP4 by JASPAR. K . qPCR analysis of the mRNA expression levels of CPT1A and BMP4 in HUVECs infected with TET2 adenovirus in presence or absence of NRF2 siRNA. n = 3/group. L . qPCR analysis of the mRNA expression levels of CPT1A and BMP4 in HUVECs treated with bardoxolone methyl in presence or absence of TET2 siRNA. n = 3/group. All data are mean ± SEM. E, Two-way ANOVA with Bonferroni post hoc test. * P < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Insulin, BMP4 and global DNA methylation levels were measured by murine INS ELISA kits (Cat#YCQZ-10385, YC Bio), murine BMP4 ELISA kits (Cat#EK0316, Boster), and Global DNA Methylation–LINE-1 Kit (Cat#55017, Active Motif) respectively.

Techniques: Binding Assay, Immunoprecipitation, Transfection, Methylation, Expressing, Infection

Journal: Egyptian Journal of Pure and Applied Science

Article Title: Assessment of serum levels of bone morphogenetic proteins as sensitive biomarkers for early prediction of acute myocardial infarction.

doi: 10.21608/ejaps.2023.188530.1051

Figure Lengend Snippet: Fig. 1 Serum levels of (A) BMP2 and (B) BMP4 in the studied groups. BMP2: Bone morphogenetic protein-2, BMP4: Bone morphogenetic protein-4.

Article Snippet: Assays for BMP2 and BMP4 Serum levels of BMP2 (Cat# CSB-E04507h) and BMP4 (Cat# CSB-E17298h) were determined according to the manufacturer’s instructions of commercial sandwich enzymelinked immunosorbent assay (ELISA) kits (Cusabio, TX, USA).

Techniques:

Journal: Egyptian Journal of Pure and Applied Science

Article Title: Assessment of serum levels of bone morphogenetic proteins as sensitive biomarkers for early prediction of acute myocardial infarction.

doi: 10.21608/ejaps.2023.188530.1051

Figure Lengend Snippet: Fig. 2 Receiver operating characteristic (ROC) curves of (A) cTnI, BMP2 and BMP4, and (B) Combined BMP2 and BMP4 to

Article Snippet: Assays for BMP2 and BMP4 Serum levels of BMP2 (Cat# CSB-E04507h) and BMP4 (Cat# CSB-E17298h) were determined according to the manufacturer’s instructions of commercial sandwich enzymelinked immunosorbent assay (ELISA) kits (Cusabio, TX, USA).

Techniques:

Journal: Egyptian Journal of Pure and Applied Science

Article Title: Assessment of serum levels of bone morphogenetic proteins as sensitive biomarkers for early prediction of acute myocardial infarction.

doi: 10.21608/ejaps.2023.188530.1051

Figure Lengend Snippet: Fig. 3 Receiver operating characteristic (ROC) curves of (A) cTnI, BMP2 and BMP4, and (B) Combined BMP2 and BMP4 to

Article Snippet: Assays for BMP2 and BMP4 Serum levels of BMP2 (Cat# CSB-E04507h) and BMP4 (Cat# CSB-E17298h) were determined according to the manufacturer’s instructions of commercial sandwich enzymelinked immunosorbent assay (ELISA) kits (Cusabio, TX, USA).

Techniques:

Journal: Egyptian Journal of Pure and Applied Science

Article Title: Assessment of serum levels of bone morphogenetic proteins as sensitive biomarkers for early prediction of acute myocardial infarction.

doi: 10.21608/ejaps.2023.188530.1051

Figure Lengend Snippet: Fig. 4 Receiver operating characteristic (ROC) curves of (A) cTnI, BMP2 and BMP4, and (B) Combined BMP2 and BMP4 to

Article Snippet: Assays for BMP2 and BMP4 Serum levels of BMP2 (Cat# CSB-E04507h) and BMP4 (Cat# CSB-E17298h) were determined according to the manufacturer’s instructions of commercial sandwich enzymelinked immunosorbent assay (ELISA) kits (Cusabio, TX, USA).

Techniques:

Journal: Egyptian Journal of Pure and Applied Science

Article Title: Assessment of serum levels of bone morphogenetic proteins as sensitive biomarkers for early prediction of acute myocardial infarction.

doi: 10.21608/ejaps.2023.188530.1051

Figure Lengend Snippet: Fig. 5 Receiver operating characteristic (ROC) curves of (A) BMP2 and (B) cTnI and BMP4 to discriminate between AMI patients without HC or DM and AMI patients with DM. AMI: Acute myocardial infarction, HC: Hypercholesterolemia, DM:

Article Snippet: Assays for BMP2 and BMP4 Serum levels of BMP2 (Cat# CSB-E04507h) and BMP4 (Cat# CSB-E17298h) were determined according to the manufacturer’s instructions of commercial sandwich enzymelinked immunosorbent assay (ELISA) kits (Cusabio, TX, USA).

Techniques: